Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: Lentiviral vectors: LV5: GP96 lentiviral vector structure; LV8: SMP30 lentiviral vector structure. (a) GP96-transfected DCs. (b) SMP30-transfected DCs. (a) and (b) show the same location.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: Plasmid Preparation, Transfection

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: WB after transfection. (a) Detection of GP96 in 6 groups. (b) Detection of SMP30 in 6 groups. (c) GAPDH as the internal control. The GP96 group expressed more GP96, and the SMP30 group expressed more SMP30, indicating that transfection was successful.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: Transfection, Control

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: Liver cancer model. (a) The liver cancer model. (b) The GP96 + SMP30 group: tumors had disappeared in some mice on day 4. (c) Tumors harvested from mice. (d) Negative TUNEL staining for apoptosis in the DC group. (e) Positive TUNEL staining for apoptosis in the GP96 + SMP30 group.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: TUNEL Assay, Staining

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: Tumor growth curve and ELISA results. Curve: the tumor volume in the GP96 + SMP30 group was smaller than that in the protein group on day 4, day 14, and day 16, P < 0.05. IL-2: the GP96 + SMP30 group showed secretion of more IL-2 than the other groups, P < 0.01. IFN- γ : the GP96 + SMP30 group showed secretion of more IFN- γ than the other groups, P < 0.01.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Healthcare Engineering

Article Title: GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma

doi: 10.1155/2022/2518847

Figure Lengend Snippet: Immunohistochemical staining of tumors. (a) Tumor expressing IL-2 in the GP96 + SMP30 group; the brown cytoplasm indicates positive staining. (b) Tumor expressing IL-2 in the GP96 group; the colorless cytoplasm indicates negative staining. (c) Tumor expressing IFN- γ in the GP96 + SMP30 group; the brown cytoplasm indicates positive staining. (d) Tumor expressing IFN- γ in the SMP30 group; the colorless cytoplasm indicates negative staining.

Article Snippet: We used mouse anti-human HSP90, rabbit anti-human GP96 (Wuhan Boster Biological Technology Ltd.), and mouse anti-human SMP30 (Santa Cruz Biotechnology Inc.) antibodies.

Techniques: Immunohistochemical staining, Staining, Expressing, Negative Staining

Journal: Open Life Sciences

Article Title: Co-downregulation of GRP78 and GRP94 Induces Apoptosis and Inhibits Migration in Prostate Cancer Cells

doi: 10.1515/biol-2019-0043

Figure Lengend Snippet: Expression of GRP78 and GRP94 in PCa and BPH tissues. A: Expression of GRP78 in BPH and PCa tissues, including a comparison of mean optical density values for GRP78 expression in each group. * indicates P<0.01 compared to BPH tissue. B: Expression of GRP94 in BPH and PCa tissues, including a comparison of mean optical density values for GRP94 expression in each group. * indicates P<0.01 compared to BPH tissue.

Article Snippet: Next, the sections were incubated overnight with anti-GRP78 and anti-GRP94 antibodies (Proteintech, USA).

Techniques: Expressing

Journal: Open Life Sciences

Article Title: Co-downregulation of GRP78 and GRP94 Induces Apoptosis and Inhibits Migration in Prostate Cancer Cells

doi: 10.1515/biol-2019-0043

Figure Lengend Snippet: Changes in GRP78 and GRP94 protein expression in PC-3 cells transfected with siGRP78, siGRP94, or siGRP78 + siGRP94. A: GRP78 and GRP94 protein expression levels in the blank, NC, siGRP78, siGRP94 and siGRP78+94 PC-3 cell groups 48 hours after transfection. B：Relative GRP78 protein expression levels in all five groups (relative to the blank group). * indicates P<0.01 compared to the NC group. C: Relative GRP94 protein expression levels in all five groups (relative to the blank group). * indicates P<0.01 compared to the NC group

Article Snippet: Next, the sections were incubated overnight with anti-GRP78 and anti-GRP94 antibodies (Proteintech, USA).

Techniques: Expressing, Transfection

Journal: Open Life Sciences

Article Title: Co-downregulation of GRP78 and GRP94 Induces Apoptosis and Inhibits Migration in Prostate Cancer Cells

doi: 10.1515/biol-2019-0043

Figure Lengend Snippet: Apoptosis rates in PC-3 cells with co-downregulation of GRP78 and GRP94. A: 48 hours after transfection, the apoptosis rates in each group were measured using Annexin V-FITC + PI double staining. The UR （upper right quadrant）+ LR（lower right quadrant） area shows apoptotic cells. B: Comparison of apoptosis rates across all five groups. * indicates P<0.01 compared to the NC group; ** indicates P<0.01 compared to the siGRP78 and siGRP94 groups.

Article Snippet: Next, the sections were incubated overnight with anti-GRP78 and anti-GRP94 antibodies (Proteintech, USA).

Techniques: Transfection, Double Staining

Journal: Open Life Sciences

Article Title: Co-downregulation of GRP78 and GRP94 Induces Apoptosis and Inhibits Migration in Prostate Cancer Cells

doi: 10.1515/biol-2019-0043

Figure Lengend Snippet: Co-downregulation of GRP78 and GRP94 inhibits PCa cell migration. A: The migratory ability of PC-3 cells in all five groups was determined using the Transwell system 48 hours post transfection. Photographs of crystal violet-stained cells that have migrated to the lower surface of the Transwell insert are shown. B: Comparison of migration inhibition rates across the NC, siGRP78, siGRP94 and siGRP78+94 groups. * indicates P<0.01 compared to the NC group; ** indicates P<0.01 compared to the siGRP78 and siGRP94 groups.

Article Snippet: Next, the sections were incubated overnight with anti-GRP78 and anti-GRP94 antibodies (Proteintech, USA).

Techniques: Migration, Transfection, Staining, Inhibition

Journal: Open Life Sciences

Article Title: Co-downregulation of GRP78 and GRP94 Induces Apoptosis and Inhibits Migration in Prostate Cancer Cells

doi: 10.1515/biol-2019-0043

Figure Lengend Snippet: Co-downregulation of GRP78 and GRP94 affects the expression of apoptosis- and migration-related proteins in PCa cells. A: 48 hours after transfection, protein expression levels of caspase-9, Bcl-2, Bax and vimentin were evaluated in PC-3 cells in the blank, NC and siGRP78+94 groups. B: Relative expression levels of the four proteins examined in panel A. Protein expression was assessed in three experimental groups and was calculated relative to the blank group. * indicates P<0.01 compared to the NC group.

Article Snippet: Next, the sections were incubated overnight with anti-GRP78 and anti-GRP94 antibodies (Proteintech, USA).

Techniques: Expressing, Migration, Transfection